The purpose of this study was to look at organizational capacity for physical activity promotion among Canadian organizations, and the influence of ParticipACTION on capacity five years after relaunch. There were mixed opinions on ParticipACTION’s contribution to capacity to promote physical activity in Canada. Phone interviews with representatives from local, provincial/territorial, and national organizations, revealed that partnerships and collaborations have strengthened physical activity promotional efforts over five years since 2007. Although information sharing technology and skilled employees have helped to maximize resources, financial infrastructure remains a key barrier to physical activity promotion.